1.0 OBJECTIVE:
To lay down a standard procedure for Proper disposal of used media plates and culture tubes.
2.0 SCOPE:
This Standard Operating Procedure is applicable to Media disposal in Microbiology Laboratory
of Quality Control Department.
3.0 RESPONSIBILITY:
Microbiologist shall be responsible to follow the procedure mention in this SOP.
4.0 ACCOUNTABILITY:
Department Head & QA Head shall be accountable for effective implementation and compliance of this SOP.
5.0 ATTACHMENTS:
Not Applicable
6.0 PROCEDURE:
6.1 Microbial media plates
6.1.1 Wear rubber hand gloves and nose mask.
6.1.2 Autoclave the Media Plates and Culture tubes Discard at 15 psi, 121°C For 30 minutes/Validation Cycle.
6.1.3 Remove media content of Petri plates with the help of spatula and collect in a Disposable polyethene In Stainless Steel Container.
6.1.4 After sterilization add 5% Dettol solution to the Media and dilute the autoclave content with tap water and drain into the sink under running tap water/ ETP plant.
6.1.5 Keep the empty glass Petri plates for 1 hour in 5% Dettol Solution or Savlon solution and then wash
the Petri plates with water and dry them for reuse. Disposable plates should be discarded after keeping them for 1 hour in 5% Dettol or Savlon solution
6.2 Discarding of Culture tubes
6.2.1 Sterilize the microbial media tubes at 15 psi 121°C for 30 minutes/ Validatioin Cycle.
6.2.2 Drain the contents of tubes into the sink under the running tap/ ETP plant.
6.2.3 Wash the empty tubes thoroughly with disinfectant and dry them for reuse.
7.0 REFERENCES:
In-house
8.0 ABBREVIATIONS:
SOP: Standard operating procedure
Psi : Pound per square inch
ETP : Effluent Treatment Plant
QA : Quality Assurance
QC: Quality Control
9.0 DISTRIBUTION LIST:
Quality Assurance
Quality Control
10.0 HISTORY OF REVISION:
| Version Number | Effective Date | Reason for Revision |
