To lay down a standard procedure for Proper disposal of used media plates and culture tubes.
This Standard Operating Procedure is applicable to Media disposal in Microbiology Laboratory
of Quality Control Department.
Microbiologist shall be responsible to follow the procedure mention in this SOP.
Department Head & QA Head shall be accountable for effective implementation and compliance of this SOP.
6.1 Microbial media plates
6.1.1 Wear rubber hand gloves and nose mask.
6.1.2 Autoclave the Media Plates and Culture tubes Discard at 15 psi, 121°C For 30 minutes/Validation Cycle.
6.1.3 Remove media content of Petri plates with the help of spatula and collect in a Disposable polyethene In Stainless Steel Container.
6.1.4 After sterilization add 5% Dettol solution to the Media and dilute the autoclave content with tap water and drain into the sink under running tap water/ ETP plant.
6.1.5 Keep the empty glass Petri plates for 1 hour in 5% Dettol Solution or Savlon solution and then wash
the Petri plates with water and dry them for reuse. Disposable plates should be discarded after keeping them for 1 hour in 5% Dettol or Savlon solution
6.2 Discarding of Culture tubes
6.2.1 Sterilize the microbial media tubes at 15 psi 121°C for 30 minutes/ Validatioin Cycle.
6.2.2 Drain the contents of tubes into the sink under the running tap/ ETP plant.
6.2.3 Wash the empty tubes thoroughly with disinfectant and dry them for reuse.
SOP: Standard operating procedure
Psi : Pound per square inch
ETP : Effluent Treatment Plant
QA : Quality Assurance
QC: Quality Control
9.0 DISTRIBUTION LIST:
10.0 HISTORY OF REVISION:
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