Description: Visual (Complies/ Not Complies)
Identification: The HPLC-chromatogram of the sample complies with standard chromatogram. (Complies/ Not Complies)
Assay: Rabeprazole sodium (20 mg) and Itopride (150 mg)
Cinematographic condition:-
Column : C18 ( 250 x 4.6 cm)
Wavelength : 280 nm
Injection volume : 20 µl
Flow rate : 1 ml/ min
Mobile phase Preparation: 600ml buffer + 400ml Acetonitrile
[Preparation of Buffer:1.46gm KH2PO4 + 525mg K2HPO4 à adjust pH 7.4 with OPA]
Procedure:-
W.S. : 23 mg Rabeprazole sodium + 150 mg Itopride in 100 ml volumetric flask add 20 ml HPLC grade methanol. sonicate for 20 min.
2 ml of above solution in 25 ml volumetric flask make up to mark with mobile phase.
Sample : Weight equivalent to Avg.fill in 100 ml Volumetric flask dissolve with 20 ml HPLC grade methanol,sonicate for 20 min. make up to mark with methanol
2 ml of above solution in 25 ml volumetric flask make up to mark with mobile phase.
Calculation :-
Rabeprazole sodium (20 mg): (90.0% to 110.0%)
Working standard weight =
Sample weight =
Working standard area =
Sample area =
Calculation:-
Test area ws wt 2 100 25 p
= ———————–X———–X————-X————X————X———-X average fill
ws area 100 25 test wt 2 100
Itopride (150 mg) : (90.0% to 110.0%)
Working standard weight =
Sample weight =
Working standard area =
Sample area =
Calculation:-
Test area ws wt 2 100 25 P
= ———————-X———–X————-X————X————X———-X Average fill
ws area 100 25 Test wt 2 100
