Assay: Each ml contains: Levosalbutamol Sulphate IP eq. to levosalbutamol 0.25 mg,
Ambroxol IP 7.5 mg & Guaiphensin IP 12.5 mg
Mobile phase preparation:[600 ml HPLC water+400ml Acetonitrile+1ml Triethylamine pH 4.0
Flow rate: 1.0ml/min
UV-Detector: 230nm
Temperature : 30 deg
Procedure:
Working standard dilution:25 mg Levosalbutamolà25 ml M.Pà 2 ml+ 60mg Ambroxol+100 mg Guaiphenesin →100ml M.P.→2ml →25ml M.P.
Sample dilution: 10mlà100ml in Mobile phase →2 ml→ 25 ml Mobile Phase
Calculation:
Weight of working standard: Sample Weight:
2 2 100 25 80
Levosalbutamol——————-X——X——–X———X————-X——–X——–X 1
25 100 25 10 2 100
= /0.25 x100= %
2 100 25
Guaiphenesin —————–X————–X——–X————–X——–X————– X 1
100 25 10 2 100
= /12.5×100= %
2 100 25
Ambroxol————————-X————–X—————X————–X———X———–X 1
100 25 10 2 100
= /7.5×100 = %
