1.0 OBJECTIVE:
To lay down a standard procedure for the calibration of Glasswares.
- SCOPE:
This SOP is applicable for calibration of Glasswares in Quality Control Laboratory
- RESPONSIBILITY:
Officers / Executive – Quality Control shall be responsible for follow that procedure.
- ACCOUNTABILITY:
Manager- Quality Control and Head QA shall be accountable for compliance of this SOP.
- ATTACHMENTS:
Calibration of Volumetric flasks Attachment-I
Calibration of Graduated pipette Attachment-I
Calibration of One mark pipette Attachment-I
Calibration of Burette Attachment-I
- PROCEDURE:
- General:
6.1.1 Certified class “A” glassware does not required the calibration.
6.1.2 For validation only class “A” glassware shall be used for analysis.
6.1.3 Calibration shall be done for all class “B” type glassware.
- Method of calibration:
- Burettes :
- Wash & rinse the burette with distilled water.
- Place a clean & dry 100 ml beaker on the balance & tare it.
- Fill the burette with distilled water temperature adjusted at 25 ± 1°C & adjust the level of the water to the zero mark while allowing the rest of the burette to become filled. Remove any air bubbles.
- Dispense into the pre-tared beaker serial aliquots of water from the filled burette. Weigh the pre-tared beaker after the addition of every aliquots of water. Take minimum three reading calculate mean value Record the result in Format-01.
- Burettes :
Calculation=Weight of water in ml = Weight of water, in gm
0.99602
Where, 0.99602 is the specific gravity of water at 25°C.
- Transfer Pipettes :
- Wash & rinse the transfer pipettes with distilled water.
- Place a clean 100 ml beaker on the balance & tare. Fill the transfer pipette with distilled water, temperature maintained at 25 ± 1°C & adjust the level of the water to the zero mark while allowing the rest of the pipettes to become filled. Remove any air bubbles.
- Dispense distilled water into the pre-tared beaker in a vertical position & then touch against the wall of the beaker. Weigh the pre-tared beaker after the addition of distilled water. Take minimum three reading & calculate mean value Record the results in Format-01.
Calculation= Weight of water, in ml = Weight of water, in gm
0.99602
Where, 0.99602 is the specific gravity of water at 25°C
- Volumetric Flask :
6.2.3.1Weigh the clean, dry, empty flask and tare the weight of flask.
- Fill the clean flask with distilled water. Set the temperature at 25°C, Reweigh it. Take minimum three reading & calculate mean value
Calculation=Weight of water in ml = Weight of water in gm
0.99602
Where, 0.99602 is the specific gravity of water at 25°C
Tolerance Chart
Volumetric Flasks: | ||||||||
Nominal capacity, ml | 5 | 10 | 25 | 50 | 100 | 250 | 500 | 1000 |
Tolerance,± | ||||||||
Class B | 0.04 | 0.04 | 0.06 | 0.08 | 0.15 | 0.2 | 0.3 | 0.8 |
One Mark Pipettes: | ||||||||
Nominal capacity, ml | 1 | 2 | 5 | 10 | 20 | |||
Tolerance,± | ||||||||
Class B | 0.02 | 0.02 | 0.03 | 0.04 | 0.05 | |||
Graduated Pipettes: | ||||||||
Nominal capacity, ml | 1 | 2 | 5 | 10 | 20 | |||
Tolerance,± | ||||||||
Class B | 0.02 | 0.06 | 0.10 | 0.15 | 0.15 | |||
Burettes: | ||||||||
Nominal capacity, ml | 25 | 50 | ||||||
Tolerance,± | ||||||||
Class B | 0.06 | 0.1 |
- REFERENCES:
In-house
- ABBREVIATIONS:
SOP: Standard Operating Procedure
QC: Quality Control
gm: Gram
ml: Milliliter
- DISTRIBUTION LIST:
Quality Assurance Department
Quality Control Department
10.0 HISTORY OF REVISION:
Version No. | Date of Revision | Reason for Revision |
Density of water: 0.99602 gm/ml | Capacity: | Tolerance:± | |||||||||
ID. No. | Temp.25°C±2°C | Frequency: | |||||||||
Sr. No. | Date | Wt. of Empty volumetric flask(A) | Wt. of volumetric with water (B) | Actual Filled volume=(B-A)/Density of water | Variation | Done by/on | Checked by/on | Remarks | Next due date | ||
Density of water: 0.99602 gm/ml | Capacity: | Tolerance:± |
ID. No. | Temp.25°C±2°C | Frequency: |
Sr. No. | Date | Wt. of Empty vessel (A) | Wt. of vessel with water (B) | Actual Filled volume=(B-A)/Density of water | Variation | Done by/on | Checked by/on | Remarks | Next due date |
Density of water: 0.99602 gm/ml | Capacity: | Tolerance:± | |||||||||
ID. No. | Temp.25°C±2°C | Frequency: | |||||||||
Sr. No. | Date | Wt. of Empty vessel (A) | Wt. of vessel with water (B) | Actual Filled volume=(B-A)/Density of water | Variation | Done by/on | Checked by/on | Remarks | Next due date | ||
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