1.0 OBJECTIVE:
To lay down a procedure for sampling and testing procedure of swab sample.
2.0 SCOPE:
This Standard Operating Procedure is applicable for sampling and testing procedure of swab sample in Microbiology Laboratory.
3.0 RESPONSIBILITY:
Microbiologist shall be responsible to follow the procedure mention in this SOP.
4.0 ACCOUNTABILITY:
Department Head & QA Head shall be accountable for effective implementation of this SOP.
5.0 ATTACHMENTS:
Report for Total Microbial Count of swab sample – Attachment I
6.0 PROCEDURE :
6.1 Prepare the sterile swab in 0.9% saline (10ml) solution in the test tube having cotton plug or use readymade sterile moisten swab (Himedia).
6.2 The swabbing shall be done on the area of 100cm2 by following horizontal strokes first followed by vertical strokes.
6.3 Place the swab back in the test tube and vortex. Incase of readymade sterile moisten
Swab pour about 10 ml of 0.9% saline in tube and vortex.
6.4 Filter the content through 0.45m membrane filter and place the filter on the Petri plate
having sterile SCDA medium.
6.5 Incubate the plates at 30- 35°C for 48 hrs and further at 20-25°C for 72 hrs.
6.6 Count the colonies and report the total microbial count as CFU/100cm2 in Attachment –I .
7.0 REFERENCES:
In- house
8.0 ABBREVIATIONS:
SOP: Standard operating procedure
CFU: Colony Forming Unit
SCDA: Soyabean Casein Digest Medium
9.0 DISTRIBUTION LIST:
Quality Assurance
Quality Control
Quality Control (Microbiology)
10.0 HISTORY OF REVISION:
| Version Number | Effective Date | Reason for Revision |
| Name of Sample :______________________________
Date of Sampling:______________________________ Date of Testing :______________________________ Date of Report :______________________________ |
Area /Location:___________________
|
|||||||||||||||||||||||
|
TOTAL MICROBIAL COUNT : (Membrane Filtration Method) For Bacteria: Medium Used : ____________________ Media Lot No.:_________________________________ Incubation Condition :Temp _______Time : From _______________to_______________ Incubator ID No.:____________________ For fungus: Medium Used : ____________________ Media Lot No.:_________________________________ Incubation Condition :Temp _______Time : From _______________to_______________
|
||||||||||||||||||||||||
| Date of Testing
|
Location ID | Observation | CFU/100cm2 | |||||||||||||||||||||
| Bacteria | Fungus | |||||||||||||||||||||||
Interpretation : Sample Complies/Does not comply .
|
||||||||||||||||||||||||
